SUMMARY REPORT OF REVIEWER COMMENTS ON THE INTERACTION PROFILE FOR ATRAZINE, DEETHYLATRAZINE, DIAZINON, NITRATE, AND SIMAZINE Submitted to: The Agency for Toxic Substances and Disease Registry

BIOSIS COPYRIGHT: BIOL ABS. C14-simazine being introduced into rats, only trace amounts oflabelled N-nitrososimazine were found in the liver, thymus, kidney and spleen tissues in single cases. Introduction of simazine together with sodium nitrite has lead to the considerable increase of the frequency of cases and amount of N-nitrososimazine formation in analyzed tissues. Nitrosation of C 14-simazine in tissues, especially liver, is much more expressed as affected by injection of the BCG vaccine than under the combined introduction of this pesticide with sodium nitrite. The results obtained indicate the possibility of simazine nitrosation and accumulation ofN-nitrososimazine in rat tissues under arrival of this pesticide into the organism together with sodium nitrite or after the induction of endogenic synthesis of nitric oxide (NO) induced by injection of the BCG vaccine into animals. This should be taken into account when estimating the danger of pesticides and other nitrogen­ containing xenobiot 41 http://toxnet.nlm.nih.gov/cgi-binisis/search/f?./temp/~AAANAaqd4: 1 :BODY 2003-05-07 B'reast Cancer and Pesticide Atrazine Bibliography BCERF Page 1 sur 1 Weisenburger, D. D., Hickman, T. l, Patil, K. D., Lawson, T. A., and Mirvish, S. S. (1990). Carcinogenesis tests of atrazine and N-nitrosoatrazine-compounds of special interest to the Midwest, Proceedings of the American Association for Cancer Research, Vol. 31 [abstract 603]. Carcinogenesis, pp. 102. 42 http://envirocancer.comell.edulBibliographylPesticidelbib.atrazine.cfm 2003-05-08 WASHINGTON, D.C. 20460 OFFICE OF PREVENTION, PESTICIDES AND TOXIC SUBSTANCES UNITED STATES ENVIRONMENTAL PROTECTION AGENCY PC Code: 080803 DATE: April 10, 2002 DP Barcode: SUBJECT: EFED Review of Public Comments in Response to the EPA EFED Revised Environmental Risk Assessment for Atrazine TO: Kimberly Lowe, Chemical Review Manager Special Review and Reregistration Division 7508C FROM: William Rabert, Biologist James Lin, Environmental Engineer Mary Frankenberry, Statistician Douglas Urban, Senior Ecologist Environmental Risk Branch 3 Environmental Fate and Effects Division 7507C THRU: Kevin Costello, Acting Chief Environmental Risk Branch 3 Environmental Fate and Effects Division 7507C The Environmental Fate and Effects Division (EFED) has reviewed many of the public comments in response to the Notice of Availability of Environmental Fate and Effects Assessment on Atrazine to Re-registration Eligibility Decision [OPP-34237A]. Comments from nine groups were considered by SRRD to address specific or general scientific issues and were referred to EFED for response. The following contains both summary and specific comments, and EFED's responses are listed below. American Water Works Association (A WWA) Comment: AWWA expressed concerns that atrazine would contaminate ground and surface water, especially sources of drinking water. EFED Response: The EFED and RED science chapters address the analysis of different studies of atrazine in surface and ground water, especially in sources of drinking water. These results and areas of uncertainty are discussed extensively. AWWA Comment: AWWA indicated that they would like to understand the Agency's rationale for coordinating the different methodologies. EFED Response: OPP and OW managers concluded that since the two offices were working